Lars Tomanek, Ph.D.
As part of the Environmental Proteomics Laboratory, I am interested in the changes in levels of protein expression in response to environmental stress. At the Environmental Proteomics Laboratory, we quantify the expression (or synthesis) levels of proteins with two-dimensional gel electrophoresis (2D GE) and identify proteins using mass spectrometry. The parallel changes in expression, so-called co-expression patterns or protein profiles, provide us with a comprehensive picture of the simultaneous changes that occur while an organism is experiencing thermal, oxidative and osmotic stress in its environment.
- How are organisms adapted to their environment
- How do they differ in their plasticity to respond to environmental change
- How do these differences in protein expression limit the ecological niche an organism can occupy
- Which proteins are the "weak links," which ones are always working the same no matter which environment the organism is found in
- What is the unity in physiological design
- What are the underlying mechanisms for the diversity of physiological systems and how do these differences determine the ecological boundaries of species distributions
Honors & Awards:
Scholander Award Finalist (American Physiological Society, 2002)
Dr. Earl H. Myers and Ethel M. Myers Grant for Oceanographic and Marine Biology, 1997
Scholar of the Studienstiftung des deutschen Volkes – German Academic National Foundation (given to the top 0.3% of the students in Germany), 1989-1994
Harley, CDG, AR Hughes, K Hultgren, BG Miner, CJB Sorte, CS Thornber, LF Rodirgues, L Tomanek and S Williams (2006): The impacts of climate change in coastal marine systems. Ecology Letters 9, 228-241.
Tomanek, L. (2005): Two-dimensional gel analysis of the heat-shock response in marine snails (genus Tegula): interspecific variation in protein expression and acclimation ability. Journal of Experimental Biology 208, 3133 - 3143.
Tomanek, L., and E. Sanford (2003). Heat-shock protein 70 (Hsp70) as a biochemical stress indicator: an experimental field test in two congeneric intertidal gastropods (genus Tegula). Biological Bulletin 205, 276 - 284.
Tomanek, L., and B. Helmuth (2002). Physiological ecology of rocky intertidal organisms: a synergy of concepts. Integrative and Comparative Biology 42, 771-775.
Tomanek, L. (2002). The heat-shock response: its variation, regulation and ecological importance in intertidal gastropods (genus Tegula). Integrative and Comparative Biology 42, 797-807.
Tomanek, L., and G. N. Somero (2002). Interspecific and acclimation-induced variation in levels of heat-shock protein 70 (hsp70) and 90 (hsp90) and heat-shock transcription factor-1 (HSF-1) in congeneric marine snails (genus Tegula): implications for regulation of hsp gene expression. Journal of Experimental Biology 205, 677-685.
Tomanek, L., and G. N. Somero (2000). Time course and magnitude of synthesis of heat-shock proteins in congeneric marine snails (genus Tegula) from different tidal heights. Physiological and Biochemical Zoology 73, 249 – 256.
Tomanek, L., and G. N. Somero (1999). Evolutionary and acclimation-induced variation in the heat-shock responses of congeneric marine snail species (genus Tegula) from different thermal habitats: implications for limits of thermotolerance and biogeography. Journal of Experimental Biology 202, 2925 - 2936.
"Physiological ecology of rocky intertidal organisms: from molecules to ecosystems", 2002 annual meeting of the Society for Integrative and Comparative Biology, Anaheim, California, 2002 (with Dr. Brian Helmuth); supported by NSF grant 0131317.
"Temperature-dependent biogeography of aquatic ectotherms: from genes to the effects of climate change", 2004 annual meeting of the German Zoological Society, Rostock (with Dr. Hans-Otto Pörtner).